Why do the silicone HPLC packing become the preferred?
< p > in the research field of high performance liquid chromatography (HPLC), for all kinds of chromatographic packing used for the separation of different objects and separation model respectively, in addition to the silica gel gradually developed the other type of packing, such as organic polymer, graphitized carbon and aluminum oxide, zirconium oxide and titanium oxide inorganic oxide, are also improved some chromatographic performance.
But there is no doubt that silica gel is still the most widely used high performance liquid chromatography (HPLC) packing.
This is because the silica gel in addition to have good mechanical strength, easy to control the pore structure and specific surface area, good chemical stability and thermal stability, and specific surface chemical reaction, and other advantages, also has a prominent advantage is its surface contains rich silicon hydroxyl, which is chemically bonded silica gel can be surface or modified on the basis of Yang.
< / p > < p > < / p > < p > as the separation material of silica gel matrix, needs to have rules of geometry, namely spherical;
The suitable particle size and narrow particle size distribution as far as possible;
Appropriate pore size and pore size distribution;
Appropriate degree of hole and specific surface area.
Spherical silica gel is more advantageous to obtain homogeneous packed bed, and make the operation of the chromatographic column pressure is low, the uniform particle size and aperture control is a very thorny problem.
Direct the preparation of the particle size of monodisperse silica microspheres is one of the goal of the chromatographic workers.
< / p > < p > < / p > < p > according to the statistics, application of siliceous fillers in packing family means account for about 80%, especially in recent years, some foreign products, such as: Germany HYPERSIL LUNA, WATERS in the United States and Britain and so on production to meet the high performance liquid chromatography (
Supercritical flow and chromatography (
Designed for the high performance of spherical silica gel, have optimized surface properties and pore structure of the rules of the as siliceous filler normally only in PH2 -
The flow of the 5 phase under the conditions of use.
Alkalinity is too big, especially in the ammonium ion, silica gel is easy to crush the dissolved;
Too much acidity, connection can learn key fracture of the organic group.
< / p > < p > < / p > < p > at present, in liquid chromatography column operation method of statistics, various types of chemical bonded stationary phase accounts for nearly 78%, almost all the traditional distribution of a liquid chromatography (HPLC) replaced by bonded phase.
That is to say, in the high performance liquid chromatography (HPLC), to use cannot be widely, a large number of solvent extraction of various silicone chemically bonded stationary phase, is another characteristic of modern liquid chromatographic packing.
< / p > < p > < / p > < p > analysis separation after is efficient separation and high detection sensitivity, and the preparation of separation should not only consider the separation efficiency, also want to consider the separation of guarantee purity the price, which strive to achieve the best performance ratio.
Separation of the separation of different purposes require different materials.
For the people care about biological engineering products required for chromatographic stationary phase or medium shall satisfy the following conditions: < / p > < p > 1.
To achieve the required separation efficiency;
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During the preparation of separation, should not cause degeneration of the detached object or inactivation: a higher recovery rate and the load;
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Chemical and mechanical properties of stable and can withstand use, clean, renewable and disinfection in bad condition.
Analysis and separation results of heavy in the sky;
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Cost is low, the price is reasonable.
< / p > < p > this paper title: silica gel HPLC packing become the preferred?
< / p > < p > recommended article read: why do you say silica gel is a kind of environmental protection material?
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